All Items
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Abstract:
Large language models predominantly rely on the Transformer architecture, whose self-attention mechanism incurs a quadratic computational cost O(N2) with respect to input length, leading to significant memory and computation bottlenecks when processing ultra-long contexts. This work proposes LanguageFold, a hierarchical sparse attention mechanism inspired by the Self-Returning Random Walk model...
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Abstract:
Glycans are essential mediators of multiple biological processes, yet their chemical complexity and non-template-driven biosynthesis pathways pose significant challenges for the establishment of the sequence-structure relationship. Here, we report cryo-EM structures of the tubular mastigoneme from a golden alga species. A large number of N- and O-glycans are observed at 1.8-2.2 Å resolutions. We...
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Abstract:
Autologous chimeric antigen receptor T (CAR-T) cell therapy has demonstrated therapeutic effectiveness in hematologic malignancies and autoimmune diseases. However, the manufacturing complexity and the required lymphodepletion hindered its wide clinical application. Engineering human T cells in vivo holds promise to conquer these limitations but requires effective T cell-targeted CAR delivery...
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Abstract:
S-PET is a compact, high sensitivity and high resolution small animal PET prototype developed at Shenzhen Bay Laboratory (SZBL). The S-PET system employs a phoswich depth of interaction (DOI) detector design, which also allows identification of the large majority of the cross layer crystal scatter (CLCS) events. This work evaluates its performance characteristics following the National Electrical...
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Abstract:
High-throughput detection of gene expression changes enables novel biological discovery. However, current methods suffer limitations. Methods coupled with RNA-sequencing involve higher costs and technological barriers while gene reporter assays only account for promoter activity. Here, we developed a high-throughput, amplification-free, and accessible method, Cellular Fluorescence Anisotropy of...
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Abstract:
While advances in omics profiling have rapidly expanded the catalog of genes associated with brain activity in health and disease, functional annotation has lagged far behind. Here, we establish a high-throughput functional genomics platform that couples the calcium-integrating sensor CaMPARI2 with CRISPRi screening in human iPSC-derived neurons. By converting cumulative neuronal activity into a...
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Abstract:
Immunity against malignant cells and the ability of cancer cells to escape anticancer immunity constitute the core process of tumor development, but the underlying mechanism is still largely unknown. Through integrated analyses of clinical samples, cellular assays, and multiple murine tumor models, our study provides compelling evidence that mitochondrial RNA (mtRNA)-derived danger signals...
Now published in Vita. doi: 10.15302/vita.2026.01.0005 -
Abstract:
Atrial fibrillation (AF) is unexpectedly prevalent in Brugada syndrome (BrS), yet the mechanisms linking SCN5A loss-of-function to atrial instability remain elusive. Here, we combined patient-specific induced pluripotent stem cell-derived atrial cardiomyocytes with label-free high-density microelectrode array (HD-MEA) mapping. We show that SCN5A haploinsufficiency creates an arrhythmogenic...
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Abstract:
Current fluorescent reporters for assessing activity of CRISPR-Cas system, such as Stoplight or split-GFP based on frameshift activation, often suffer from limitations including large vector size, low viral packaging efficiency, and insensitivity to in-frame insertions or deletions. Although specialized reporters for base editing (e.g., BEAR) and prime editing (e.g., PEAR and fluoPEER) have been...
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Abstract:
Understanding how molecular binding couples with diffusion is fundamental to molecular engineering and biomedical applications. At the mesoscale, this coupling gives rise to a binding memory effect, characterized by power-law decays in temporal binding autocorrelations. This repeated binding-unbinding cycles transforms the static, affinity-limited binding picture into a dynamic landscape. While...